One of four FPs: cyan (eCFP), green (sGFP), yellow (eYFP) or the mCherry variant of red FP (RFP), with a fused mitochondrial targeting sequence, was introduced to carrot cell lines of three varieties using Agrobacterium-mediated transformation.
This paper describes the non-toxic fluorescent protein (FP) tagging of protoplasts which allows discrimination of fusion components and identification of hybrids in real-time during electrofusion. This process can be induced by the application of electric current to isolated protoplasts, but such electrofusion requires an effective hybrid cell identification method. Both games implemented an unusual storytelling technique, gradually uncovering past events by encouraging the player to look for messages and logs left by people before the disasters occurred.Somatic hybridisation in the carrot, as in other plant species, enables the development of novel plants with unique characteristics. The player could customize and enhance the player's parameters, affecting gameplay style and offering diverse solutions to the tasks.
System Shock 2 (1999) further emphasized the role-playing angle, effectively becoming a FPS/RPG hybrid. Objectives, character customization, and interaction with the game world exceeded by far the customary requirements of contemporary shooters, infusing the game with role-playing elements. Though similar to other first-person shooters in terms of basic gameplay mechanics, the game's pace was nevertheless quite different, requiring the player to carefully explore the environment, collecting clues and crucial items in process. Similarly to that game, System Shock strived to represent realistic physical interaction in a 3D world. Released in 1994, System Shock was based on the engine implemented in Ultima Underworld. Related by plot (including a common antagonist, the malevolent artificial intelligence known as SHODAN), the games share similar science fiction horror scenarios, in which the protagonist is trapped in an abandoned, enclosed environment (space station, space ship, etc.), trying to fend off enemies (cyborgs, mutated creatures, and others), find clues that would reveal past events, survive, and escape. System Shock games were notable for incorporating elements of first-person shooter, puzzle-solving, and role-playing.
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